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mouse antihuman antibody il 6  (InvivoGen)


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    Structured Review

    InvivoGen mouse antihuman antibody il 6
    Mouse Antihuman Antibody Il 6, supplied by InvivoGen, used in various techniques. Bioz Stars score: 94/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+antihuman+antibody+il+6/Anti-hIL-6-IgG/pm37191536-202-34-41
    Average 94 stars, based on 21 article reviews
    mouse antihuman antibody il 6 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Bacterial Lipoproteins Shift Cellular Metabolism to Glycolysis in Macrophages Causing Bone Erosion.
    Article Snippet: .. For the blocking assay in MM6 cells, 5 105 cells were incubated for 1 h with either 1 mg/mL of rabbit anti-human antibodies TNF (catalog no. D1B4; Cell Signaling, Leiden, Netherlands), 1 mg/mL of mouse antihuman antibody IL-6 (catalog no. mabg-hil6-3; InvivoGen, Toulouse, France), 1 mg/mL of rat antihuman antibody IL-10 (catalog no. AHC0103; Thermo Fisher, Schwerte, Germany), or 1 mg/mL mouse anti-human monoclonal antibody IgG1 (catalog no. bgal-mab1-ctrlm; InvivoGen, Toulouse, France) as isotype control. ..

    Incubation:

    Article Title: Bacterial Lipoproteins Shift Cellular Metabolism to Glycolysis in Macrophages Causing Bone Erosion.
    Article Snippet: .. For the blocking assay in MM6 cells, 5 105 cells were incubated for 1 h with either 1 mg/mL of rabbit anti-human antibodies TNF (catalog no. D1B4; Cell Signaling, Leiden, Netherlands), 1 mg/mL of mouse antihuman antibody IL-6 (catalog no. mabg-hil6-3; InvivoGen, Toulouse, France), 1 mg/mL of rat antihuman antibody IL-10 (catalog no. AHC0103; Thermo Fisher, Schwerte, Germany), or 1 mg/mL mouse anti-human monoclonal antibody IgG1 (catalog no. bgal-mab1-ctrlm; InvivoGen, Toulouse, France) as isotype control. ..

    Control:

    Article Title: Bacterial Lipoproteins Shift Cellular Metabolism to Glycolysis in Macrophages Causing Bone Erosion.
    Article Snippet: .. For the blocking assay in MM6 cells, 5 105 cells were incubated for 1 h with either 1 mg/mL of rabbit anti-human antibodies TNF (catalog no. D1B4; Cell Signaling, Leiden, Netherlands), 1 mg/mL of mouse antihuman antibody IL-6 (catalog no. mabg-hil6-3; InvivoGen, Toulouse, France), 1 mg/mL of rat antihuman antibody IL-10 (catalog no. AHC0103; Thermo Fisher, Schwerte, Germany), or 1 mg/mL mouse anti-human monoclonal antibody IgG1 (catalog no. bgal-mab1-ctrlm; InvivoGen, Toulouse, France) as isotype control. ..



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    <t>Blocking</t> <t>IL-6</t> abolishes degenerative IVD induced elevated neuron activity in response to mechanical stimulation. A, The percentage of rat DRG neurons exhibiting calcium transients as a function of strain fitted to a Boltzman curve for the control media, degenerative IVD conditioned media and degenerative IVD conditioned media supplemented with IL-6 blocking antibody (n = 5 patients) groups. B, The maximum calcium transients of rat DRG neurons exposed to mechanical strain in the presence of control media, degenerative IVD conditioned media, and degenerative IVD conditioned media supplemented with IL-6 blocking antibody. C,D, The S50 (C) and Smax (D) generated from dose-response curve fitting for the control media, degenerative IVD conditioned media, and degenerative IVD conditioned media supplemented with IL-6 blocking antibody. A, Values are mean ± SD. *P < .05 compared to control media group at same strain level. Error bars are SD. B-D, Values are mean ± SD. *P < .05 compared to control media group. Error bars are SD. DRG, dorsal root ganglia; IL, interleukin; IVD, intervertebral disc
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    Image Search Results


    Blocking IL-6 abolishes degenerative IVD induced elevated neuron activity in response to mechanical stimulation. A, The percentage of rat DRG neurons exhibiting calcium transients as a function of strain fitted to a Boltzman curve for the control media, degenerative IVD conditioned media and degenerative IVD conditioned media supplemented with IL-6 blocking antibody (n = 5 patients) groups. B, The maximum calcium transients of rat DRG neurons exposed to mechanical strain in the presence of control media, degenerative IVD conditioned media, and degenerative IVD conditioned media supplemented with IL-6 blocking antibody. C,D, The S50 (C) and Smax (D) generated from dose-response curve fitting for the control media, degenerative IVD conditioned media, and degenerative IVD conditioned media supplemented with IL-6 blocking antibody. A, Values are mean ± SD. *P < .05 compared to control media group at same strain level. Error bars are SD. B-D, Values are mean ± SD. *P < .05 compared to control media group. Error bars are SD. DRG, dorsal root ganglia; IL, interleukin; IVD, intervertebral disc

    Journal: Journal of orthopaedic research : official publication of the Orthopaedic Research Society

    Article Title: Degenerative IVD conditioned media and acidic pH sensitize sensory neurons to cyclic tensile strain

    doi: 10.1002/jor.24682

    Figure Lengend Snippet: Blocking IL-6 abolishes degenerative IVD induced elevated neuron activity in response to mechanical stimulation. A, The percentage of rat DRG neurons exhibiting calcium transients as a function of strain fitted to a Boltzman curve for the control media, degenerative IVD conditioned media and degenerative IVD conditioned media supplemented with IL-6 blocking antibody (n = 5 patients) groups. B, The maximum calcium transients of rat DRG neurons exposed to mechanical strain in the presence of control media, degenerative IVD conditioned media, and degenerative IVD conditioned media supplemented with IL-6 blocking antibody. C,D, The S50 (C) and Smax (D) generated from dose-response curve fitting for the control media, degenerative IVD conditioned media, and degenerative IVD conditioned media supplemented with IL-6 blocking antibody. A, Values are mean ± SD. *P < .05 compared to control media group at same strain level. Error bars are SD. B-D, Values are mean ± SD. *P < .05 compared to control media group. Error bars are SD. DRG, dorsal root ganglia; IL, interleukin; IVD, intervertebral disc

    Article Snippet: Based on previous experience with IVD conditioned media, 32 mouse monoclonal antihuman IL-6 antibody (Life Technologies) was added to degenerative IVD conditioned media and incubated at 37°C for three hours prior to addition to neurons as described in the Degenerative IVD Conditioned Media Exposure section ( n = 5).

    Techniques: Blocking Assay, Activity Assay, Generated

    Blocking IL-6 abolishes degenerative IVD induced elevated neuron activity in response to mechanical stimulation. A, The percentage of rat DRG neurons exhibiting calcium transients as a function of strain fitted to a Boltzman curve for the control media, degenerative IVD conditioned media and degenerative IVD conditioned media supplemented with IL-6 blocking antibody (n = 5 patients) groups. B, The maximum calcium transients of rat DRG neurons exposed to mechanical strain in the presence of control media, degenerative IVD conditioned media, and degenerative IVD conditioned media supplemented with IL-6 blocking antibody. C,D, The S50 (C) and Smax (D) generated from dose-response curve fitting for the control media, degenerative IVD conditioned media, and degenerative IVD conditioned media supplemented with IL-6 blocking antibody. A, Values are mean ± SD. *P < .05 compared to control media group at same strain level. Error bars are SD. B-D, Values are mean ± SD. *P < .05 compared to control media group. Error bars are SD. DRG, dorsal root ganglia; IL, interleukin; IVD, intervertebral disc

    Journal: Journal of orthopaedic research : official publication of the Orthopaedic Research Society

    Article Title: Degenerative IVD conditioned media and acidic pH sensitize sensory neurons to cyclic tensile strain

    doi: 10.1002/jor.24682

    Figure Lengend Snippet: Blocking IL-6 abolishes degenerative IVD induced elevated neuron activity in response to mechanical stimulation. A, The percentage of rat DRG neurons exhibiting calcium transients as a function of strain fitted to a Boltzman curve for the control media, degenerative IVD conditioned media and degenerative IVD conditioned media supplemented with IL-6 blocking antibody (n = 5 patients) groups. B, The maximum calcium transients of rat DRG neurons exposed to mechanical strain in the presence of control media, degenerative IVD conditioned media, and degenerative IVD conditioned media supplemented with IL-6 blocking antibody. C,D, The S50 (C) and Smax (D) generated from dose-response curve fitting for the control media, degenerative IVD conditioned media, and degenerative IVD conditioned media supplemented with IL-6 blocking antibody. A, Values are mean ± SD. *P < .05 compared to control media group at same strain level. Error bars are SD. B-D, Values are mean ± SD. *P < .05 compared to control media group. Error bars are SD. DRG, dorsal root ganglia; IL, interleukin; IVD, intervertebral disc

    Article Snippet: IL-6 blocking experiments Based on previous experience with IVD conditioned media, 32 mouse monoclonal antihuman IL-6 antibody (Life Technologies) was added to degenerative IVD conditioned media and incubated at 37°C for three hours prior to addition to neurons as described in the Degenerative IVD Conditioned Media Exposure section ( n = 5).

    Techniques: Blocking Assay, Activity Assay, Generated